Drug & Alcohol Testing — Louisville, KY
Drug & Alcohol Testing
in Louisville, KY
DOT-compliant and workplace drug testing programs — lab-based, instant, and breath alcohol — serving Louisville, KY and surrounding areas.
Explore Our Testing Services
Why Louisville Chooses Elite Testing Labs
Walk In — No Appointment
Located at 5312 S 3rd St #103 in South Louisville (40214). Just walk in during business hours — no scheduling required.
Same-Day Results Available
Instant drug testing provides negative results in about 10 minutes. Lab-based results are typically ready within 24–72 hours.
Fentanyl Testing Available
Our 12-panel and 14-panel tests include fentanyl screening — something many Louisville testing centers do not offer.
Professional Employer Testing
Professional testing solutions for employers, schools, court requirements, and personal testing needs. Transparent pricing on every panel.
Select Your Drug or Alcohol Test
Browse every test we offer. Click any row to see exactly what's screened, how collection works, and how to register — no appointment needed.
DOT / Federal testing
Required for CDL drivers, trucking, aviation & transportation
Employment drug testing (lab-based)
Certified lab • 24–72 hr results • Legally defensible • MRO review included
Instant / rapid testing (same-day results)
Results within 10 minutes · Non-negative = sent to lab automatically · No extra charge ever
Alcohol testing
DOT and non-DOT breath alcohol — certified BAT technician — immediate results
What to Expect When You Walk In
Understand the process before you walk in
LAB-BASED TESTING
INSTANT / RAPID TESTING
Frequently Asked Questions
Common Questions About Drug & Alcohol Testing in Louisville
Everything you need to know before you walk in — from pricing and panels to MRO review and chain of custody.
Still have questions? Call us at (502) 205-1299 — we're happy to help.
Find Us in South Louisville
Conveniently located in South Louisville — serving the greater Louisville, KY area including 40214, 40215, and surrounding zip codes.
Office Hours
Walk-ins: Mon–Thu 12pm–7:30pm · Fri 2:30pm–7:30pm
Appointments: Mon–Fri 7am–12pm · Sat by appt
📞 Phone available 24/7
Phone
(502) 205-1299Need testing at your workplace? We offer mobile drug testing — we come to you.